Lrrc49tm1b(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Lrrc49tm1b(EUCOMM)Wtsi |
Name: |
leucine rich repeat containing 49; targeted mutation 1b, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5646003 |
Gene: |
Lrrc49 Location: Chr9:60494507-60595460 bp, - strand Genetic Position: Chr9, 32.75 cM
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Alliance: |
Lrrc49tm1b(EUCOMM)Wtsi page
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IMPC: |
Lrrc49 gene page |
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Mutant Cell Line: |
EPD0618_5_H10 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 60528631 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 60529428. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding this knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:155845, J:173534, J:188991)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
5 reference(s) |
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