Col1a1^{tm1(tetO-GFP/RNAi:Sirt1)Pyc}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Sirt1)Pyc}
• Name: collagen, type I, alpha 1; targeted mutation 1, Peter Y Chuang
• MGI ID: MGI:5646012
• Synonyms: Col-TGM, Sirt1^RNAi
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Sirt1)Pyc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213867
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Knockdown, RMCE-ready, Reporter)
• Mutation: Insertion
• Col1a1^{tm1(tetO-GFP/RNAi:Sirt1)Pyc} involves 1 genes/genome features (Sirt1) View all
• Mutation details: RMCE on KH2 cells inserted a cassette that contains the tetracycline promoter and a GFP fusion with a Mir30e-based short hairpin sequence targeting Sirt1 (Sirt1.688) into the 3'UTR. (J:213867)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:213867 Chuang PY, et al., In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells. Am J Pathol. 2014 Jul;184(7):1940-56
• All: 2 reference(s)