Tfap2a^tm2.1Will
Targeted Allele Detail

Summary

• Symbol: Tfap2a^tm2.1Will
• Name: transcription factor AP-2, alpha; targeted mutation 2.1, Trevor Williams
• MGI ID: MGI:5647993
• Synonyms: NEO, Neoflox
• Gene: Tfap2a Location: Chr13:40868778-40891852 bp, - strand Genetic Position: Chr13, 20.01 cM, cytoband A5-B1
• Alliance: Tfap2a^tm2.1Will page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88826
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 5 and an frt-flanked neo cassette was inserted after exon 6, followed by another loxP site. RT-PCR analysis indicates that homozygotes contain only 35% of wild-type Tfap2a levels and RNA analysis indicates that the allele generates aberrant transcripts. (J:88826)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tfap2a Mutation: 39 strains or lines available

References

• Original: J:88826 Brewer S, et al., Wnt1-Cre-mediated deletion of AP-2alpha causes multiple neural crest-related defects. Dev Biol. 2004 Mar 1;267(1):135-52
• All: 4 reference(s)