Sik1^tm1.2Berd
Targeted Allele Detail

Summary

• Symbol: Sik1^tm1.2Berd
• Name: salt inducible kinase 1; targeted mutation 1.2, Rebecca Berdeaux
• MGI ID: MGI:5648836
• Gene: Sik1 Location: Chr17:32063224-32074778 bp, - strand Genetic Position: Chr17, 17.25 cM
• Alliance: Sik1^tm1.2Berd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236223
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted between exons 1 and 2. An FRT-flanked neomycin resistance cassette with a loxP site was inserted between exons 7 and 8. Flp-mediated recombination remove the selection cassette. Cre-mediated recombination removed exons 2 through 7. Western blot analysis confirmed the absence of protein expression in primary hepatocytes. (J:236223)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sik1 Mutation: 41 strains or lines available

References

• Original: J:236223 Nixon M, et al., Skeletal muscle salt inducible kinase 1 promotes insulin resistance in obesity. Mol Metab. 2016 Jan;5(1):34-46
• All: 1 reference(s)