Kras^tm4.1Bbd
Targeted Allele Detail

Summary

• Symbol: Kras^tm4.1Bbd
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 4.1, Mariano Barbacid
• MGI ID: MGI:5649285
• Synonyms: K-Ras^V14I
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm4.1Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223433
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site flanked hygromycin resistance gene and STOP cassette was inserted 100 bp upstream of a Kras mutagenized exon 2 in which valine codon 14 (GTA) was changed to isoleucine (ATA) (p.V14I). Cre-mediated recombination in the germline removed the floxed LSL cassette, allowing expression of the mutant allele. (J:223433)

Disease models

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm4.1Bbd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:223433 Hernandez-Porras I, et al., K-RasV14I recapitulates Noonan syndrome in mice. Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16395-400
• All: 3 reference(s)