Fos^tm1.1Fawa
Targeted Allele Detail

Summary

• Symbol: Fos^tm1.1Fawa
• Name: FBJ osteosarcoma oncogene; targeted mutation 1.1, Fan Wang
• MGI ID: MGI:5660357
• Synonyms: Fos^TVA
• Gene: Fos Location: Chr12:85520664-85524047 bp, + strand Genetic Position: Chr12, 39.7 cM
• Alliance: Fos^tm1.1Fawa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242828
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The targeting vector contains a 2A sequence, followed by a destabilized nuclear CFP (nuclear localized CFP fused with "PEST" sequence), 2A sequence, followed by destabilized TVA (TVA fused with "PEST" sequence), bGH polyA. An FRT-flanked PGK-NEO cassette was inserted adjacent to the STOP codon in exon 4, of the Fos gene. "PEST" sequence targets the protein for degradation by fusing amino acids 422-462 from the degradation domain of mouse ornithine decarboxylase to the C-terminal of the reporter/protein (in this case CFP and TVA). (J:242828)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fos Mutation: 43 strains or lines available

References

• Original: J:242828 Sakurai K, et al., Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit. Neuron. 2016 Nov 23;92(4):739-753
• All: 11 reference(s)