Igs2^{tm1(CAG-mt-Keima)Fink}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-mt-Keima)Fink}
• Name: intergenic site 2; targeted mutation 1, Toren Finkel
• MGI ID: MGI:5660493
• Synonyms: mt-Keima
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-mt-Keima)Fink} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270139
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The construct contains the chicken beta-actin promoter (with cytomegalovirus immediate early enhancer) driving expression of the mitochondrial targeting sequence from Cox8a (cytochrome c oxidase subunit VIIIa) fused to a pH-sensitive fluorescent protein, Keima, flanked by attB sites. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from Igs2^tm3.1Luo mice. The Igs2^tm3.1Luo allele contains three tandem attP sites. The PhiC31 integrase catalyzes recombination between the attB and attP sites. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Igs2^tm3.1Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺)-derived R1 ES cells.

References

• Original: J:270139 Sun N, et al., Measuring In Vivo Mitophagy. Mol Cell. 2015 Nov 19;60(4):685-96
• All: 27 reference(s)