Col1a1^{tm4(CAG-FGFR2_iIIIb*K660N)Kkw}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(CAG-FGFR2_iIIIb*K660N)Kkw}
• Name: collagen, type I, alpha 1; targeted mutation 4, Kwok-Kin Wong
• MGI ID: MGI:5661319
• Synonyms: FGFR2^K660N
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(CAG-FGFR2_iIIIb*K660N)Kkw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214230
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Col1a1^{tm4(CAG-FGFR2_iIIIb*K660N)Kkw} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FGFR2 (2263)

  Fgfr2

  (MGI:95523)

• Mutation details: The chicken beta-actin (CAG) promoter, a loxP flanked STOP cassette, and human FGFR2 isoform IIIb cDNA with the kinase K660N domain activating mutation were inserted into the mouse collagen A1 locus. Upon cre-mediated recombination, the STOP cassette is excised promoting expression of the mutant FGFR2. (J:214230)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:214230 Tchaicha JH, et al., Kinase domain activation of FGFR2 yields high-grade lung adenocarcinoma sensitive to a Pan-FGFR inhibitor in a mouse model of NSCLC. Cancer Res. 2014 Sep 1;74(17):4676-84
• All: 1 reference(s)