Agl^tm1Geno
Targeted Allele Detail

Summary

• Symbol: Agl^tm1Geno
• Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase; targeted mutation 1, Genoway
• MGI ID: MGI:5661387
• Gene: Agl Location: Chr3:116533648-116601815 bp, - strand Genetic Position: Chr3, 50.47 cM
• Alliance: Agl^tm1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218471
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 32-34 (coding for the last 114 amino acids) were replaced with a loxP flanked neomycin resistance cassette. This deletes the carboxy terminus, including the glucosidase and glycogen-binding domains. Western blot analysis confirmed absence of protein in all tissues analyzed. (J:218471)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agl Mutation: 83 strains or lines available

References

• Original: J:218471 Pagliarani S, et al., Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Nov;1842(11):2318-28
• All: 2 reference(s)