Tg(Cd4-BCL2*G101A*D102A*D103A)AWnt
Transgene Detail

Summary

• Symbol: Tg(Cd4-BCL2*G101A*D102A*D103A)AWnt
• Name: transgene insertion A, Astar Winoto
• MGI ID: MGI:5661474
• Synonyms: BH3 Tg
• Transgene: Tg(Cd4-BCL2*G101A*D102A*D103A)AWnt Location: unknown
• Alliance: Tg(Cd4-BCL2*G101A*D102A*D103A)AWnt page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cd4-BCL2*G101A*D102A*D103A)AWnt expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2 (596)

  Bcl2	(MGI:88138)

  Expresses human BCL2 with amino acids 101-103 mutated from glycine-aspartate-aspartate to alanine-alanine-alanine

• Mutation details: The human BCL2 with amino acids 101-103 mutated from glycine-aspartate-aspartate to alanine-alanine-alanine to abolish BH3 domain pro-apoptotic function is under the control of the T cell-specific Cd4 locus control region (LCR). Immunohistochemistry confirmed expression of the transgene in thymocytes. A compensatory downregulation of endogenous mouse Bcl2 in the thymus, lymph nodes, and spleen is seen with transgene expression. Two lines were chosen for analysis, A and B, with similar phenotypes. (J:217939)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:217939 Burger ML, et al., T cell-specific inhibition of multiple apoptotic pathways blocks negative selection and causes autoimmunity. Elife. 2014;3
• All: 1 reference(s)