Dcpp1^{tm1.1(cre/ERT2)Ovi}
Targeted Allele Detail

Summary

• Symbol: Dcpp1^{tm1.1(cre/ERT2)Ovi}
• Name: demilune cell and parotid protein 1; targeted mutation 1.1, Catherine E Ovitt
• MGI ID: MGI:5661581
• Synonyms: Dcpp1-GCE, Dcpp1^GCE, Dcpp1-GFPCreErT2, Dcpp1^{tm1(cre/ERT2)Ovi}, Dcpp1^tm1(GCE)Ovi
• Gene: Dcpp1 Location: Chr17:24099850-24101827 bp, + strand Genetic Position: Chr17, 12.11 cM
• Alliance: Dcpp1^{tm1.1(cre/ERT2)Ovi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229520
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Dcpp1^{tm1.1(cre/ERT2)Ovi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dcpp1 (MGI:105949)

• Mutation details: The EGFP-cre/ERT2 (GCE) fragment with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct interrupted coding sequence in exon 2 and placed the GCE gene under the control of the endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. No expression of GFP was detected with fluorescence or using antibody to GFP. The fusion cre is expressed in intercalated duct cells of parotid gland and serous cells of sublingual gland. (J:229520)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dcpp1 (mouse) Summary of all recombinase alleles driven by Dcpp1. Comparative matrix view of recombinase activities Alliance mouse Dcpp1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dcpp1 Mutation: 5 strains or lines available

Notes

No evidence of ectopic expression in lungs, kidney, pancreas, or ovaries.

References

• Original: J:229520 Maruyama EO, et al., Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands. PLoS One. 2016;11(1):e0146711
• All: 2 reference(s)