Comt^{tm2(COMT)Xzho}
Targeted Allele Detail

Summary

• Symbol: Comt^{tm2(COMT)Xzho}
• Name: catechol-O-methyltransferase; targeted mutation 1, Xianjin Zhou
• MGI ID: MGI:5661843
• Synonyms: Human COMT Met
• Gene: Comt Location: Chr16:18225636-18245602 bp, - strand Genetic Position: Chr16, 11.4 cM
• Alliance: Comt^{tm2(COMT)Xzho} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224284
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Comt^{tm2(COMT)Xzho} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  COMT (1312)

  Comt	(MGI:88470)

  The human sequence is modified to contain the amino acid substitution Val158Met.

• Mutation details: The targeting construct was designed to replace the entire endogenous coding region with the human sequence following by a neomycin (neo) resistance cassette. A missense mutation was introduced in exon 3, corresponding with human codon 158, resulting in the Val158Met mutation commonly associated with schizophrenia and other psychiatric disorders. Western blot analysis confirmed the absence of protein expression in the hippocampus and cortex. (J:224284)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Comt Mutation: 24 strains or lines available

References

• Original: J:224284 Risbrough V, et al., Generation and characterization of humanized mice carrying COMT158 Met/Val alleles. Neuropsychopharmacology. 2014 Jul;39(8):1823-32
• All: 2 reference(s)