Ddr2^{tm1.1(cre/Esr1*)Rcow}
Targeted Allele Detail

Summary

• Symbol: Ddr2^{tm1.1(cre/Esr1*)Rcow}
• Name: discoidin domain receptor family, member 2; targeted mutation 1.1, Randy T Cowling
• MGI ID: MGI:5662369
• Synonyms: Ddr2^null
• Gene: Ddr2 Location: Chr1:169799876-169938331 bp, - strand Genetic Position: Chr1, 76.84 cM, cytoband H1-H5
• Alliance: Ddr2^{tm1.1(cre/Esr1*)Rcow} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214642
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Ddr2^{tm1.1(cre/Esr1*)Rcow} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ddr2 (MGI:1345277)

• Mutation details: Part of exon 3 was replaced with two mutated mouse estrogen receptors that sequester cre to the cytoplasm, polyadenylation sequence and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in cultured cardiac fibroblasts. Only low expression of the modified cre product was detected. (J:214642)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ddr2 (mouse) Summary of all recombinase alleles driven by Ddr2. Alliance mouse Ddr2 gene page

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddr2 Mutation: 61 strains or lines available

References

• Original: J:214642 Cowling RT, et al., Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse. Am J Physiol Heart Circ Physiol. 2014 Sep 1;307(5):H773-81
• All: 4 reference(s)