Tfcp2l1^{tm1.1(cre/ERT2)Ovi}
Targeted Allele Detail

Summary

• Symbol: Tfcp2l1^{tm1.1(cre/ERT2)Ovi}
• Name: transcription factor CP2-like 1; targeted mutation 1.1, Catherine E Ovitt
• MGI ID: MGI:5662395
• Synonyms: Tcfcp2l1^GCE, Tcf-GCE, Tcf^tm1(GCE)Ovi, Tfcp2l1^{tm1(cre/ERT2)Ovi}
• Gene: Tfcp2l1 Location: Chr1:118555675-118612898 bp, + strand Genetic Position: Chr1, 52.14 cM, cytoband E2
• Alliance: Tfcp2l1^{tm1.1(cre/ERT2)Ovi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229520
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Tfcp2l1^{tm1.1(cre/ERT2)Ovi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tfcp2l1 (MGI:2444691)

• Mutation details: The targeting construct was generated by inserting the diphtheria toxin A gene (DTA, for positive selection), 3.2 kb 5 homologous arm containing the 5' UTR of exon 1, and 5.5 kb 3 homologous arm containing exon 2 into pBluescript SKII(+). Then the eGFP-CreERT2 (GCE) fragment (McMahon et al. 2008) with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. (J:229520)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tfcp2l1 (mouse) Summary of all recombinase alleles driven by Tfcp2l1. Comparative matrix view of recombinase activities Alliance mouse Tfcp2l1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tfcp2l1 Mutation: 99 strains or lines available

References

• Original: J:229520 Maruyama EO, et al., Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands. PLoS One. 2016;11(1):e0146711
• All: 5 reference(s)