Rtcb^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rtcb^{tm1c(KOMP)Wtsi}
• Name: RNA 2',3'-cyclic phosphate and 5'-OH ligase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5688394
• Synonyms: Rtcb^fl
• Gene: Rtcb Location: Chr10:85774501-85793657 bp, - strand Genetic Position: Chr10, 41.81 cM
• Alliance: Rtcb^{tm1c(KOMP)Wtsi} page
• IMPC: Rtcb gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216740
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 85786952 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 85787741. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:216740)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rtcb Mutation: 25 strains or lines available

Notes

Cell line EPD0044_1_G09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:216740

References

• Original: J:216740 Jurkin J, et al., The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells. EMBO J. 2014 Dec 17;33(24):2922-36
• All: 1 reference(s)