Snx10^{tm1a(EUCOMM)Raba}
Targeted Allele Detail

Summary

• Symbol: Snx10^{tm1a(EUCOMM)Raba}
• Name: sorting nexin 10; targeted mutation 1a, Ricardo A Battaglino
• MGI ID: MGI:5690193
• Synonyms: Snx10^Neo-f
• Gene: Snx10 Location: Chr6:51500882-51567659 bp, + strand Genetic Position: Chr6, 24.95 cM, cytoband B3
• Alliance: Snx10^{tm1a(EUCOMM)Raba} page

Snx10^{tm1a(EUCOMM)Raba}/Snx10^{tm1a(EUCOMM)Raba} mice are growth retarded, have a tooth eruption defect and have bones that lack marrow cavities

Show the 9 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224711
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

Allele Type: Targeted (Conditional ready, Hypomorph, Reporter) Mutation: Insertion Mutation details: The L1L2_Bact_P cassette (vector PG00216_Z_2_C06) was inserted at position 51556250 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 51557821. The critical exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. PCR indicates that mRNA expression is bone is reduced by about 86%. (J:224711)

Generation of the Snx10^tm1a(EUCOMM)Raba allele

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Snx10 Mutation: 142 strains or lines available

References

• Original: J:224711 Ye L, et al., Osteopetrorickets due to Snx10 deficiency in mice results from both failed osteoclast activity and loss of gastric acid-dependent calcium absorption. PLoS Genet. 2015 Mar;11(3):e1005057
• All: 2 reference(s)