Emc8^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Emc8^{tm1b(EUCOMM)Hmgu}
• Name: ER membrane protein complex subunit 8; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5692625
• Gene: Emc8 Location: Chr8:121380653-121394826 bp, - strand Genetic Position: Chr8, 69.98 cM
• Alliance: Emc8^{tm1b(EUCOMM)Hmgu} page
• IMPC: Emc8 gene page

Mutation origin

• Mutant Cell Line: HEPD0711_2_B02
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Emc8^{tm1a(EUCOMM)Hmgu} allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Emc8^tm1b(EUCOMM)Hmgu/Emc8^tm1b(EUCOMM)Hmgu	C57BL/6N-Emc8^tm1b(EUCOMM)Hmgu/H	HEPD0711_2_B02
ht2	Emc8^tm1b(EUCOMM)Hmgu/Emc8^+	C57BL/6N-Emc8^tm1b(EUCOMM)Hmgu/H	HEPD0711_2_B02

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms Sex:
Source:	IMPC - HAR	IMPC - HAR	IMPC - HAR
adipose tissue		√
increased total body fat amount		√
cardiovascular system			√
abnormal retina blood vessel morphology			√
enlarged heart			√
growth/size/body		√	√
enlarged heart			√
decreased lean body mass		√
hematopoietic system			√
increased red blood cell distribution width			√
increased neutrophil cell number			√
immune system			√
increased neutrophil cell number			√
mortality/aging	√
preweaning lethality, complete penetrance	√
vision/eye			√
abnormal retina blood vessel morphology			√
mydriasis			√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	57 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Emc8 Mutation: 18 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 3 reference(s)