Alg14^{tm2b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Alg14^{tm2b(EUCOMM)Hmgu}
• Name: asparagine-linked glycosylation 14; targeted mutation 2b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5692664
• Synonyms: Alg14^-
• Gene: Alg14 Location: Chr3:121085422-121156743 bp, + strand Genetic Position: Chr3, 52.94 cM
• Alliance: Alg14^{tm2b(EUCOMM)Hmgu} page
• IMPC: Alg14 gene page

Alg14^{tm2b(EUCOMM)Hmgu}/Alg14^{tm2b(EUCOMM)Hmgu} mice exhibit embryonic lethality, with embryos recovered at E3.5 as blastocysts but no embryos at E7.5. Blastocysts fail to hatch from the zona pellucida an appear necrotic after 72 hours in culture.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: HEPD0817_2_A10
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Alg14^{tm2a(EUCOMM)Hmgu} allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739)

Expression

In Mice Carrying this Mutation:	46 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alg14 Mutation: 11 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 4 reference(s)