Tbc1d24^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tbc1d24^{tm1b(EUCOMM)Hmgu}
• Name: TBC1 domain family, member 24; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5692831
• Synonyms: Tbc1d24^tm1b
• Gene: Tbc1d24 Location: Chr17:24394405-24424536 bp, - strand Genetic Position: Chr17, 12.3 cM
• Alliance: Tbc1d24^{tm1b(EUCOMM)Hmgu} page
• IMPC: Tbc1d24 gene page

Mutation origin

• Mutant Cell Line: HEPD0549_1_A08
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Tbc1d24^{tm1a(EUCOMM)Hmgu} allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons (exons 3-5) leaving behind the inserted lacZ reporter sequence. qRT-PCR and Western blot analysis revealed a significant (~50%) reduction of RNA and protein expression in the hippocampus of heterozygous mutant mice at P15. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739, J:271632)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d24 Mutation: 82 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 3 reference(s)