Hdac6^tm1.1Tyao
Targeted Allele Detail

Summary

• Symbol: Hdac6^tm1.1Tyao
• Name: histone deacetylase 6; targeted mutation 1.1, Tso-Pang Yao
• MGI ID: MGI:5693117
• Gene: Hdac6 Location: ChrX:7796359-7814128 bp, - strand Genetic Position: ChrX, 3.58 cM
• Alliance: Hdac6^tm1.1Tyao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128914
• Parent Cell Line: TC (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 10, an FRT-flanked neomycin resistance (neo) and zeocin (Zeo) resistance cassette, followed by a second loxP site downstream of exon 13. Cre-mediated recombination removed the floxed cassette. Immunoblot analysis of brain samples demonstrated the absence of protein, (J:128914)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hdac6 Mutation: 17 strains or lines available

Notes

The prinicpal investigator has confirmed the that the original construct included loxP sites flanking exons 10-13 and an FRT-flanked neo/zeo cassette. Cre-mediated recombination removed exons 10-13.

References

• Original: J:128914 Gao YS, et al., Histone deacetylase 6 regulates growth factor-induced actin remodeling and endocytosis. Mol Cell Biol. 2007 Dec;27(24):8637-47
• All: 17 reference(s)