Kpnb1^tm1.2Mfz
Targeted Allele Detail

Summary

• Symbol: Kpnb1^tm1.2Mfz
• Name: karyopherin subunit beta 1; targeted mutation 1.2, Mike Fainzilber
• MGI ID: MGI:5694123
• Gene: Kpnb1 Location: Chr11:97050540-97078707 bp, - strand Genetic Position: Chr11, 60.95 cM, cytoband D
• Alliance: Kpnb1^tm1.2Mfz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222342
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into exon 22 downstream of the STOP codon. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted into exon 22 downstream of the 1.1 kb sequence to be deleted from the 3' UTR, followed by three SV40 polyA signals. Flp-mediated recombination removed the selection cassette and cre-mediated recombination deleted the 1.1 kb sequence in the 3' UTR. RT-PCR confirmed the absence of transcripts with an intact 3' UTR. (J:222342)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kpnb1 Mutation: 57 strains or lines available

References

• Original: J:222342 Perry RB, et al., Subcellular knockout of importin beta1 perturbs axonal retrograde signaling. Neuron. 2012 Jul 26;75(2):294-305
• All: 3 reference(s)