Sema6a^{tm2b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sema6a^{tm2b(EUCOMM)Hmgu}
• Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; targeted mutation 2b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5694672
• Gene: Sema6a Location: Chr18:47378321-47504267 bp, - strand Genetic Position: Chr18, 25.08 cM, cytoband C
• Alliance: Sema6a^{tm2b(EUCOMM)Hmgu} page
• IMPC: Sema6a gene page

Mutation origin

• Mutant Cell Line: HEPD0769_4_E07
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 47423916 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 47425538. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and neomycin selection cassette yielding this knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065, J:188991)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema6a Mutation: 85 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)