Kcnmb2^tm1.1Clin
Targeted Allele Detail

Summary

• Symbol: Kcnmb2^tm1.1Clin
• Name: potassium large conductance calcium-activated channel, subfamily M, beta member 2; targeted mutation 1.1, Christopher J Lingle
• MGI ID: MGI:5694679
• Synonyms: beta2^floxed, kcnmb2 floxed
• Gene: Kcnmb2 Location: Chr3:31956656-32254329 bp, + strand Genetic Position: Chr3, 15.48 cM
• Alliance: Kcnmb2^tm1.1Clin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225860
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 1 and a second loxP site downstream of exon 1 followed by an FRT-flanked neomycin resistance (neo) cassette which included a third loxP site, downstream of exon 1. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 1 floxed. (J:225860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnmb2 Mutation: 17 strains or lines available

References

• Original: J:225860 Martinez-Espinosa PL, et al., Knockout of the BK beta2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. J Gen Physiol. 2014 Oct;144(4):275-95
• All: 2 reference(s)