Srsf2^tm1.1Oaw
Targeted Allele Detail

Summary

• Symbol: Srsf2^tm1.1Oaw
• Name: serine and arginine-rich splicing factor 2; targeted mutation 1.1, Omar Abdel-Wahab
• MGI ID: MGI:5694699
• Synonyms: Srsf2P95H
• Gene: Srsf2 Location: Chr11:116740727-116743920 bp, - strand Genetic Position: Chr11, 81.49 cM
• Alliance: Srsf2^tm1.1Oaw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221404
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 1, cDNA encoding the sequence of exon 3 fused in frame to the endogenous exon 2, followed by an SV40 polyadenylation sequence bound to an FRT-flanked neomycin resistance (neo) cassette and a second loxP site. Duplicate exons 1-2 were inserted downstream of the second loxP site and upstream of the endogenous exon 3. The second exon 1 contains a mutation in amino acid 95 which alters the nucleotide sequence from CCG to CAC, resulting in an amino acid change from Proline to Histidine. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:221404)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Srsf2^tm1.1Oaw

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Srsf2 Mutation: 11 strains or lines available

References

• Original: J:221404 Kim E, et al., SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition. Cancer Cell. 2015 May 11;27(5):617-30
• All: 10 reference(s)