St3gal4^tm1.1Bsi
Targeted Allele Detail

Summary

• Symbol: St3gal4^tm1.1Bsi
• Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 4; targeted mutation 1.1, RIKEN Brain Science Institute
• MGI ID: MGI:5695539
• Synonyms: ST3Gal IV-KO
• Gene: St3gal4 Location: Chr9:34957872-35028160 bp, - strand Genetic Position: Chr9, 19.36 cM, cytoband A5.3
• Alliance: St3gal4^tm1.1Bsi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217104
• Parent Cell Line: MS12 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted in intron 2 and an FRT flanked pgk-neo cassette in inverse orientation and loxP site were inserted in intron 3 via homologous recombination. Cre mediated recombination removed exon 3 and the neo cassette. Quantitative real-time PCR analysis confirmed the absence of expression in homozygous mutant mice. (J:217104)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any St3gal4 Mutation: 26 strains or lines available

References

• Original: J:217104 Srimontri P, et al., Sialyltransferase ST3Gal IV deletion protects against temporal lobe epilepsy. J Neurochem. 2014 Dec;131(5):675-87
• All: 3 reference(s)