Ccl27a^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Ccl27a^{tm1.1(KOMP)Vlcg}
• Name: C-C motif chemokine ligand 27A; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5695899
• Synonyms: CCL27a^-, CCL27a-KO
• Gene: Ccl27a Location: Chr4:41769467-41774247 bp, - strand Genetic Position: Chr4, 22.08 cM
• Alliance: Ccl27a^{tm1.1(KOMP)Vlcg} page
• IMPC: Ccl27a gene page

Mutation origin

• Mutant Cell Line: 14813A-F9
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: Cre-mediated excision of the parental Ccl27a^{tm1(KOMP)Vlcg} allele resulted in the removal of the neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. The coding sequence for the Ccl27a gene is deleted, whereas its duplicate genes Ccl27b and Ccl27al (aka Gm13306) remain intact. Immunofluorescence staining and real-time RT-PCR analyses still detected a significant level of CCL27 expression in the skin of homozygous mutant mice as a result of Ccl27b and/or Ccl27al expression. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739, J:330550)

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccl27a Mutation: 13 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 3 reference(s)