Supt6^{Gt(fVET5A)Hiir}
Gene trapped Allele Detail

Summary

• Symbol: Supt6^{Gt(fVET5A)Hiir}
• Name: SPT6, histone chaperone and transcription elongation factor; gene trap fVET5A, Takashi Hiiragi
• MGI ID: MGI:5696412
• Synonyms: Supt6^Gt
• Gene: Supt6 Location: Chr11:78097575-78136798 bp, - strand Genetic Position: Chr11, 46.74 cM
• Alliance: Supt6^{Gt(fVET5A)Hiir} page

Mutation origin

• Strain of Origin: (C57BL/6 x C3H)F2

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The lentiviral gene trap vector sinLV-pRRL-K contains a gene trapping splice acceptor; stop codons in all three reading frames; an internal ribosomal entry site (IRES); the Venus reporter gene with a nuclear localization signal; a woodchuck hepatitis post-transcriptional regulatory element (WPRE); central poly-purine tract (cPPT); Rev-responsive element (RRE); and Rous sarcoma virus promoter (RSV) all flanked by a self-inactivating lentiviral long terminal (LTR) consisting of U5, R and truncated U3 element (delta U3). The gene trap vector inserted into exon 10. Western blot analysis confirmed the absence of protein expression. (J:224530)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Supt6 Mutation: 68 strains or lines available

References

• Original: J:224530 Dietrich JE, et al., Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages. EMBO Rep. 2015 Aug;16(8):1005-21
• All: 1 reference(s)