D130043K22Rik^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: D130043K22Rik^{tm1b(KOMP)Wtsi}
• Name: RIKEN cDNA D130043K22 gene; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5696928
• Synonyms: Kiaa0319-NZ
• Gene: D130043K22Rik Location: Chr13:25029118-25085253 bp, + strand Genetic Position: Chr13, 10.72 cM
• Alliance: D130043K22Rik^{tm1b(KOMP)Wtsi} page
• IMPC: D130043K22Rik gene page

Mutation origin

• Mutant Cell Line: EPD0077_5_D04
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25047100 of Chromosome 13 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 25047964. Exon 6 is thus flanked by loxP sites. Subsequent cre-mediated recombination deleted exon 6 and the neo cassette, creating a reporter knockout allele. (J:204739)

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any D130043K22Rik Mutation: 57 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 6 reference(s)