Evc2^tm2.1Mis
Targeted Allele Detail

Summary

• Symbol: Evc2^tm2.1Mis
• Name: EvC ciliary complex subunit 2; targeted mutation 2.1, Yuji Mishina
• MGI ID: MGI:5697358
• Synonyms: Evc2/Limbin conditional mice, floxP, fx
• Gene: Evc2 Location: Chr5:37495843-37582399 bp, + strand Genetic Position: Chr5, 20.15 cM
• Alliance: Evc2^tm2.1Mis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226455
• Parent Cell Line: A3-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to introduce one loxP site and an FRT-PGKneo-FRT cas- sette to intron 12 and the other loxP site to intron 14, leaving exons 13 and 14 flanked by the loxP sites. F1 offspring were bred with Flp deleter mice to remove the neo cassette through recombination at the FRT site. (J:226455)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Evc2 Mutation: 47 strains or lines available

References

• Original: J:226455 Zhang H, et al., Generation of Evc2/Limbin global and conditional KO mice and its roles during mineralized tissue formation. Genesis. 2015 Jul 28
• All: 5 reference(s)