Pcdh15^tm3.1Mll
Targeted Allele Detail

Summary

• Symbol: Pcdh15^tm3.1Mll
• Name: protocadherin 15; targeted mutation 3.1 Ulrich Muller
• MGI ID: MGI:5697809
• Synonyms: deltaCD3, exon39KO, PCDH15-deltaCD3
• Gene: Pcdh15 Location: Chr10:72935174-74485569 bp, + strand Genetic Position: Chr10, 37.43 cM
• Alliance: Pcdh15^tm3.1Mll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171627
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: A Frt-flanked neomycin selection cassette replaced exon 39. The pGK-neo cassette was subsequently removed by crossing the mice carrying the targeted allele to Flp-deleter mice. qPCR and in situ hybridization analyses of P1 homozygous mutant mice confirmed that expression of the CD3 isoform is abolished while expression of isoforms CD1 and CD2 is maintained. However, expression of CD2 is upregulated and CD2 is found to be ectopically expressed in support cells and Kollikers organ, rather than being restricted to IHCs and OHCs as in wild-type controls. (J:171627)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdh15 Mutation: 133 strains or lines available

References

• Original: J:171627 Webb SW, et al., Regulation of PCDH15 function in mechanosensory hair cells by alternative splicing of the cytoplasmic domain. Development. 2011 Apr;138(8):1607-17
• All: 2 reference(s)