Kdm2a^{tm1d(KOMP)Mtok}
Targeted Allele Detail

Summary

• Symbol: Kdm2a^{tm1d(KOMP)Mtok}
• Name: lysine (K)-specific demethylase 2A; targeted mutation 1d, Makio Tokunaga
• MGI ID: MGI:5697958
• Synonyms: Fbxl11^null
• Gene: Kdm2a Location: Chr19:4366172-4448749 bp, - strand Genetic Position: Chr19, 4.01 cM
• Alliance: Kdm2a^{tm1d(KOMP)Mtok} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217589
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The KOMP targeting vector PG00184_Z_8_C04 inserted an FRT site, lacZ reporter gene, loxP site, neomycin resistance cassette, FRT site and loxP site upstream of exon 7 and a third loxP site downstream of exon 9. Flp-mediated recombination removed the reporter gene and selection cassette. Cre-mediated recombination removed exons 7 through 9. Western blot analysis confirmed the absence of protein expression in E10.5 embryos. (J:217589)

Expression

In Mice Carrying this Mutation:	52 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm2a Mutation: 103 strains or lines available

References

• Original: J:217589 Kawakami E, et al., The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators. Mech Dev. 2015 Feb;135:31-42
• All: 1 reference(s)