Scube2^tm1Rbya
Targeted Allele Detail

Summary

• Symbol: Scube2^tm1Rbya
• Name: signal peptide, CUB domain, EGF-like 2; targeted mutation 1, Ruey-Bing Yang
• MGI ID: MGI:5698363
• Synonyms: Scube2^Flox
• Gene: Scube2 Location: Chr7:109397897-109464886 bp, - strand Genetic Position: Chr7, 57.53 cM
• Alliance: Scube2^tm1Rbya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226676
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: Two loxP sites were inserted sequentially into the gene separated by 65 kb of genomic sequence, flanking exons 2 to 21. The 5 and 3 neomycin resistance gene cassettes were removed by transfection of the correctly targeted ES cells with a Cre- or Flp-recombinase encoding expression plasmid, respectively. The final targeted loxP-flanked allele contains a loxP site in the first intron and a FRT/loxP site downstream in the 21st intron of the gene. (J:226676)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scube2 Mutation: 41 strains or lines available

References

• Original: J:226676 Lin YC, et al., Disruption of Scube2 Impairs Endochondral Bone Formation. J Bone Miner Res. 2015 Jul;30(7):1255-67
• All: 3 reference(s)