Scube2^tm1.1Rbya
Targeted Allele Detail

Summary

• Symbol: Scube2^tm1.1Rbya
• Name: signal peptide, CUB domain, EGF-like 2; targeted mutation 1.1, Ruey-Bing Yang
• MGI ID: MGI:5698367
• Gene: Scube2 Location: Chr7:109397897-109464886 bp, - strand Genetic Position: Chr7, 57.53 cM
• Alliance: Scube2^tm1.1Rbya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226676
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre-mediated recombination of Scube2^tm1Rbya resulted in a 65kb deletion encompassing exons 2-21 of the gene. The deleted region contains the coding sequences for nine tandem epidermal growth factor-like domains, the spacer region, three cysteine-rich repeats, and the CUB domain of the encoded protein. The deletion also introduces a reading frame shift. RT-PCR with primer pairs specific for the wild-type mRNA detected the transcript in MEFs and in multiple tissues, at the highest levels in heart, kidney and testis, of wild-type mice, but no expression in any tissue analyzed from homozygous mutant mice. This was confirmed for the protein in heart, lung and testis by western blot analysis. (J:226676)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scube2 Mutation: 41 strains or lines available

References

• Original: J:226676 Lin YC, et al., Disruption of Scube2 Impairs Endochondral Bone Formation. J Bone Miner Res. 2015 Jul;30(7):1255-67
• All: 1 reference(s)