Baz1a^tm1.1Sky
Targeted Allele Detail

Summary

• Symbol: Baz1a^tm1.1Sky
• Name: bromodomain adjacent to zinc finger domain 1A; targeted mutation 1.1, Scott Keeney
• MGI ID: MGI:5698832
• Synonyms: Baz1a^-
• Gene: Baz1a Location: Chr12:54940336-55061133 bp, - strand Genetic Position: Chr12, 23.84 cM, cytoband C
• Alliance: Baz1a^tm1.1Sky page

Morphological defects of Baz1a^tm1.1Sky/Baz1a^tm1.1Sky sperm

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223044
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: LoxP sites were inserted to flank exon six, then exon 6 was deleted via Cre-mediated recombination. An FRT-flanked neomycin selection cassette was also inserted next to the loxP site in intron 5 and is retained in the final allele. The encoded protein was undetectable in homozygous animals by immunoblot of whole testis extracts, western blot of extracts of ear fibroblasts, or immunostaining of testis sections or spermatocyte squashes. (J:223044)

Generation of the Baz1a^tm1.1Sky and Baz1a^tm1Sky alleles

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Baz1a Mutation: 82 strains or lines available

References

• Original: J:223044 Dowdle JA, et al., Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. PLoS Genet. 2013 Nov;9(11):e1003945
• All: 1 reference(s)