Gt(ROSA)26Sor^{tm1.1(CAG-Venus,-Sdk2)Jrs}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-Venus,-Sdk2)Jrs}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Joshua R Sanes
• MGI ID: MGI:5698913
• Synonyms: Rosa-CAG-LOX-STOP-LOX-Venus-3F2A-Sidekick2-WPRE-FRT-neo-FRT, VG3^swap
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-Venus,-Sdk2)Jrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225578
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-Venus,-Sdk2)Jrs} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sdk2 (MGI:2443847)

• Mutation details: The targeting vector inserted into the locus a CAG promoter, floxed STOP sequence, Venus reporter gene, tripleF2A (three tandem repeats of foot-and-mouth disease 2A peptide sequence), Sdk2 gene, WPRE sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the neomycin resistance cassette. (J:225578)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:225578 Krishnaswamy A, et al., Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27;524(7566):466-70
• All: 2 reference(s)