Prdm9^tm1.1Kpgn
Targeted Allele Detail

Summary

• Symbol: Prdm9^tm1.1Kpgn
• Name: PR domain containing 9; targeted mutation 1.1, Kenneth Paigen
• MGI ID: MGI:5699103
• Synonyms: Prdm9^CAST-KI, Prdm9^Cst, Prdm9^dom2H, Prdm9^TmHu
• Gene: Prdm9 Location: Chr17:15763341-15784616 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Prdm9^tm1.1Kpgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226896
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J/J

Mutation description

• Allele Type: Targeted
• Mutation: Other
• Mutation details: Exon 12, encoding the zinc finger domain, and 55bp of flanking sequence from intron 11 and 106bp of flanking sequence from the 3-prime UTR from the CAST/EiJ sequence of Prdm9 was inserted in place of that of the endogenous sequence in B6(Cg)-Tyr/J derived ES Cells and an FRT flanked neomycin selection cassette was inserted 621bp downstream of the end of exon 12. Breeding to a Cre deleter strain excised the neo cassette. (J:226896)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm9 Mutation: 46 strains or lines available

References

• Original: J:226896 Baker CL, et al., PRDM9 binding organizes hotspot nucleosomes and limits Holliday junction migration. Genome Res. 2014 May;24(5):724-32
• All: 7 reference(s)