Alk^tm1.1Rhp
Targeted Allele Detail

Summary

• Symbol: Alk^tm1.1Rhp
• Name: anaplastic lymphoma kinase; targeted mutation 1.1, Ruth H Palmer
• MGI ID: MGI:5700375
• Synonyms: ALK KO
• Gene: Alk Location: Chr17:72175967-72911622 bp, - strand Genetic Position: Chr17, 43.77 cM
• Alliance: Alk^tm1.1Rhp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226955
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A NLS-lacZ-polyA cassette was inserted into intron 19. It was flanked on the 5'end with a single FRT site and on the 3' end with a single loxP site. An additional loxP site followed by another FRT site was inserted into intron 23. Flp-mediated recombination in ES cells deleted the expression cassette and exons 20-23 (i.e. the kinase domain encoding exons). Western blot confirmed that no detectable protein is expressed from this allele. (J:226955)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alk Mutation: 64 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Hypogonadotropic Hypogonadism in male homozygotes (J:226955)

References

• Original: J:226955 Witek B, et al., Targeted Disruption of ALK Reveals a Potential Role in Hypogonadotropic Hypogonadism. PLoS One. 2015;10(5):e0123542
• All: 1 reference(s)