Gt(ROSA)26Sor^{tm4(CAG-mOrange2,-EGFP,-mKate2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-mOrange2,-EGFP,-mKate2)Zjh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Z Josh Huang
• MGI ID: MGI:5700411
• Synonyms: RGBow
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-mOrange2,-EGFP,-mKate2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235123
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), and three farnesylated fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites. Specifically, the coding region contains (from 5' to 3') a loxP site, a lox2272 site, a loxN site, a transcriptional STOP signal, mOrange2 sequence (derived from a monomeric red fluorescent protein) with polyA sequence, a loxP site, EGFP (enhanced green fluorescent protein) with polyA sequence, a lox2272 site, mKate2 (derived from a monomeric far-red fluorescent protein). This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:235123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:235123 He M, et al., Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron. 2016 Sep 21;91(6):1228-1243
• All: 3 reference(s)