Gata6^tm1Hadj
Targeted Allele Detail

Summary

• Symbol: Gata6^tm1Hadj
• Name: GATA binding protein 6; targeted mutation 1, Anna-Katerina Hadjantonakis
• MGI ID: MGI:5700508
• Synonyms: Gata6^H2B-Venus, Gata6^{tm1(HIST1H2BB/Venus)Hadj}
• Gene: Gata6 Location: Chr18:11052510-11085636 bp, + strand Genetic Position: Chr18, 5.63 cM
• Alliance: Gata6^tm1Hadj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226600
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to insert downstream of the first non-coding intron with (from 3' to 5') an FRT site, engrailed 2 intron and exon with splice acceptor site, H2B-Venus-polyadenylation sequence, rox-flanked beta actin promoter driven NEO cassette, and a loxP site, followed by exon 2 and a second loxP site. Two alternative translation initiation codons are present in exon 2. (J:226600, J:227467)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gata6 Mutation: 35 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351).

References

• Original: J:226600 Freyer L, et al., A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice. BMC Dev Biol. 2015;15:38
• All: 3 reference(s)