About   Help   FAQ
Vcam1tm2Dmil
Targeted Allele Detail
Summary
Symbol: Vcam1tm2Dmil
Name: vascular cell adhesion molecule 1; targeted mutation 2, David Milstone
MGI ID: MGI:5700541
Synonyms: VcamkB
Gene: Vcam1  Location: Chr3:115903669-115923337 bp, - strand  Genetic Position: Chr3, 50.17 cM
Alliance: Vcam1tm2Dmil page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:227109
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Not Applicable)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsSequential tag and exchange gene targeting was used to introduce a 6 nucleotide mutation in the 5NF-B binding site of the transcriptional promoter, and a 3 nucleotide mutation in the 5UTR of Vcam1 in exon 1. The first, "tag", targeting vector replaces exon 1 (encoding the signal peptide) and part of exon 2 (encoding immunoglobulin-like domain 1) with a pgk-neomycin/TK cassettes. The Tag construct is transfected into embryonic stems cells and a single correctly targeted clone is transfected with the second construct. The second "Exchange" targeting construct replaces the neo cassette and introduces a 6 nucleotide substitution 5' of the transcription start site resulting in the disruption of the 5'NFKB1 (NF-kappaB) binding site domain and a 3 nucleotide substitution downstream of the translation start site in the 5' UTR of exon 1. The 3 nt substitution is used to distinguish the mutant and wild-type mRNA and hnRNA. Western blot ananlysis demonstrates the presence of mutant protein in heart endothelial cells. (J:227109)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Vcam1 Mutation:  44 strains or lines available
References
Original:  J:227109 Milstone DS, et al., Differential role of an NF-kappaB transcriptional response element in endothelial versus intimal cell VCAM-1 expression. Circ Res. 2015 Jul 3;117(2):166-77
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory