Ift80^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ift80^{tm1c(KOMP)Wtsi}
• Name: intraflagellar transport 80; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5701373
• Synonyms: IFT80^flox
• Gene: Ift80 Location: Chr3:68799832-68911892 bp, - strand Genetic Position: Chr3, 32.07 cM, cytoband E2
• Alliance: Ift80^{tm1c(KOMP)Wtsi} page
• IMPC: Ift80 gene page

Mutation origin

• Mutant Cell Line: EPD0356_2_D09
• Germline Transmission: Earliest citation of germline transmission: J:226948
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 68874929 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 68875710. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:226948)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ift80 Mutation: 56 strains or lines available

References

• Original: J:226948 Omori Y, et al., Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control. PLoS One. 2015;10(6):e0128422
• All: 1 reference(s)