Gt(ROSA)26Sor^{tm1(CAG-Mir182)Dgk}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Mir182)Dgk}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David Kirsch
• MGI ID: MGI:5702434
• Synonyms: LSL-miR-182
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Mir182)Dgk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217635
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Mir182)Dgk} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir182 (MGI:2676846)

• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), miR-182 sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:217635)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:217635 Sachdeva M, et al., MicroRNA-182 drives metastasis of primary sarcomas by targeting multiple genes. J Clin Invest. 2014 Oct;124(10):4305-19
• All: 2 reference(s)