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Gt(ROSA)26Sortm2.1(CAG-EGFP,-DTA*G128D)Pjen
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm2.1(CAG-EGFP,-DTA*G128D)Pjen
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Patricia Jensen
MGI ID: MGI:5702438
Synonyms: RC::L-DTA
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm2.1(CAG-EGFP,-DTA*G128D)Pjen page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:252681
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Mutation detailsThe targeting vector iss designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a rox-flanked His3-SV40 transcriptional STOP cassette, a frt-flanked His3-SV40 transcriptional STOP cassette, a cre-dependent FLEx switch (containing [from 5' to 3'] an inward-facing lox2272 site, a cDNA sequence encoding enhanced green fluorescent protein [eGFP], a rabbit -globin polyA signal, an inward-facing loxP site, a reverse complimentary sequence encoding the tox 176 attenuated diphtheria toxin A chain [G-to-A transition at nt 383 encodes glycine-to-aspartic acid at amino acid 128 (DTA*G128D)], a second inward-facing lox2272 site, a second inward-facing loxP site ), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. This entire targeting vector is inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. A PhiC31-expressing plasmid is used to remove the attB/attP-flanked PGK-FRT-Neo-polyA cassette and replace it with the recombined attB/attP site (attL). Flp-mediated recombination removed the FRT-flanked STOP sequence. Dre-mediated recombination removed the rox-flanked STOP sequence. The resulting allele has a CAG hybrid promoter followed by a variant lox-flanked region containing an eGFP cassette and an inverted attenuated diptheria toxin subunit alpha gene (DTA*G128D). GFP expression is visualized by direct fluorescence in embryos and adult brain. (J:252681)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 2 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  992 strains or lines available
References
Original:  J:252681 Plummer NW, et al., A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch. Genesis. 2017 Oct;55(10)
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory