Gt(ROSA)26Sor^{tm2.1(CAG-EGFP,-DTA*G128D)Pjen}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-EGFP,-DTA*G128D)Pjen}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Patricia Jensen
• MGI ID: MGI:5702438
• Synonyms: RC::L-DTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-EGFP,-DTA*G128D)Pjen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252681
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector iss designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a rox-flanked His3-SV40 transcriptional STOP cassette, a frt-flanked His3-SV40 transcriptional STOP cassette, a cre-dependent FLEx switch (containing [from 5' to 3'] an inward-facing lox2272 site, a cDNA sequence encoding enhanced green fluorescent protein [eGFP], a rabbit -globin polyA signal, an inward-facing loxP site, a reverse complimentary sequence encoding the tox 176 attenuated diphtheria toxin A chain [G-to-A transition at nt 383 encodes glycine-to-aspartic acid at amino acid 128 (DTA*G128D)], a second inward-facing lox2272 site, a second inward-facing loxP site ), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. This entire targeting vector is inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. A PhiC31-expressing plasmid is used to remove the attB/attP-flanked PGK-FRT-Neo-polyA cassette and replace it with the recombined attB/attP site (attL). Flp-mediated recombination removed the FRT-flanked STOP sequence. Dre-mediated recombination removed the rox-flanked STOP sequence. The resulting allele has a CAG hybrid promoter followed by a variant lox-flanked region containing an eGFP cassette and an inverted attenuated diptheria toxin subunit alpha gene (DTA*G128D). GFP expression is visualized by direct fluorescence in embryos and adult brain. (J:252681)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:252681 Plummer NW, et al., A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch. Genesis. 2017 Oct;55(10)
• All: 5 reference(s)