Tg(RasE)290Biat
Transgene Detail

Summary

• Symbol: Tg(RasE)290Biat
• Name: transgene insertion 290, Biomodels Austria
• MGI ID: MGI:5702681
• Synonyms: Multi-Hit, Ras effector (RasE) Multi-Hit transgene, RasE Multi-Hit transgene
• Transgene: Tg(RasE)290Biat Location: unknown
• Alliance: Tg(RasE)290Biat page

Transgene origin

• Strain of Origin: C57BL/6 or DBA/2

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(RasE)290Biat expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hras

• Mutation details: The "RasE Multi-Hit BAC" (bacterial artificial chromosome) includes three independent expression modules, each containing the CAGGS promoter (comprising the cytomegalovirus immediate early (CMV-IE) enhancer and the chicken beta-actin promoter, first exon and first intron, with the 3' splice junction from the rabbit beta-globin gene); a mutant Hras gene combining the G12V (glycine to valine at amino acid (aa) 12) mutation with either T35S (threonine to serine at aa 35) (Hras^V12S35), E37G (glutamate to glycine at aa 37) (Hras^V12G37), or Y40C (tyrosine to cysteine at aa 40) (Hras^V12C40); an internal ribosomal entry site; a fluorescent reporter gene (eYFP, dsRed, or hCD2t, respectively); and an SV40 polyadenylation signal; each module flanked by chicken 2xHS4 insulator sequences. This construct was recombined into the PstI restriction site of a BAC containing the mouse collagen, type I, alpha 1 (Col1a1) locus. Transgenic mouse line 290 was shown by Southern blot analysis to have integrated a single copy of the BAC. (J:226601)

The "RasE Multi-Hit BAC" transgene construct

Tumor Data

List all tumor models in MMHCdb carrying Tg(RasE)290Biat

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgenic construct was microinjected into (C57BL/6 x DBA/2) fertilized oocytes. (J:226601)

References

• Original: J:226601 Musteanu M, et al., A mouse model to identify cooperating signaling pathways in cancer. Nat Methods. 2012 Sep;9(9):897-900
• All: 1 reference(s)