Ccn2^{tm1.1(folA/cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Ccn2^{tm1.1(folA/cre)Hze}
• Name: cellular communication network factor 2; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5702703
• Synonyms: Ctgf-2A-dgCre, Ctgf-2A-dgCre-D
• Gene: Ccn2 Location: Chr10:24471340-24474581 bp, + strand Genetic Position: Chr10, 11.84 cM, cytoband A3-B1
• Alliance: Ccn2^{tm1.1(folA/cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227758
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Ccn2^{tm1.1(folA/cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ccn2 (MGI:95537)

• Mutation details: The targeting vector contains, from 5' to 3', a partial exon 1 through intron 4 sequence containing an FRT3 site in intron 4, a partial exon 5 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the coding sequence, a dgCre fusion gene (described below) that is in-frame with the coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an FRT5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dgCre fusion gene is an enhanced green fluorescent protein/Cre recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31 integrase-mediated recombination removed the AttB/AttP-flanked sequences. Trimethoprim-treatment is required to stabilize the cre protein. No EGFP is detected with or without induction. (J:227758)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ccn2 (mouse) Summary of all recombinase alleles driven by Ccn2. Alliance mouse Ccn2 gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community The donating investigator reports that Ctgf-2A-dgCre-D mice exhibit TMP-inducible Cre recombinase activity that is specific and efficient (largely recapitulates the endogenous Ctgf expression pattern with efficient inducibility). When induced, strong Cre recombinase activity is restricted to layer 6b of the cortex and in restricted populations within the cortical subplate (e.g., endopiriform nucleus). In the absence of TMP, Cre activity is only present in a few cortical layer 6b cells.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccn2 Mutation: 29 strains or lines available

References

• Original: J:227758 Tasic B, et al., Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nat Neurosci. 2016 Feb;19(2):335-46
• All: 13 reference(s)