Rock2^tm1.1Itl
Targeted Allele Detail

Summary

• Symbol: Rock2^tm1.1Itl
• Name: Rho-associated coiled-coil containing protein kinase 2; targeted mutation 1.1, inGenious Targeting Laboratory
• MGI ID: MGI:5702750
• Synonyms: Rock2^flox
• Gene: Rock2 Location: Chr12:16944896-17037824 bp, + strand Genetic Position: Chr12, 8.04 cM, cytoband A3
• Alliance: Rock2^tm1.1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222206
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv or C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A single loxP site was inserted 5' to exon 3 and a loxP/FRT-flanked neomycin resistance cassette was inserted 3' to exon 3 via homologous recombination. Flp-mediated recombination deleted the neo cassette leaving exon 3 floxed. (J:222206)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rock2 Mutation: 60 strains or lines available

Notes

"The targeting construct was linearized and electroporated into C57BL/6 129/SvEv hybrid embryonic stem cells (inGenious Targeting Laboratory)." (J:222206)

References

• Original: J:222206 Shimizu T, et al., Crucial role of ROCK2 in vascular smooth muscle cells for hypoxia-induced pulmonary hypertension in mice. Arterioscler Thromb Vasc Biol. 2013 Dec;33(12):2780-91
• All: 1 reference(s)