Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Catherine D Van Raamsdonk
• MGI ID: MGI:5702870
• Synonyms: Gt(ROSA)26Sor^{tm1(GNAQ*)Cvr}, Rosa26-fs-GNAQ-Q209L
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225597
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GNAQ (2776)

  Gnaq	(MGI:95776)

  The mutant form of GNAQ expressed is constitutively active by substitution of Glutamine 209 for Leucine (Q209L).

• Mutation details: A targeting vector was designed with a minimal adenovirus type 2 major late splice acceptor, a loxP-flanked Neo-STOP cassette, a cDNA sequence encoding human GNAQ* (a mutant form of GNAQ rendered constitutively active by substitution of Glutamine 209 for Leucine (Q209L), and a bovine growth hormone polyadenylation signal. (J:225597)

Disease models

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:225597 Huang JL, et al., Oncogenic G Protein GNAQ Induces Uveal Melanoma and Intravasation in Mice. Cancer Res. 2015 Aug 15;75(16):3384-97
• All: 5 reference(s)