Tg(Cck-EGFP/RNAi:Gad1)2Mirn
Transgene Detail
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Symbol: |
Tg(Cck-EGFP/RNAi:Gad1)2Mirn |
Name: |
transgene insertion 2, Karoly Mirnics |
MGI ID: |
MGI:5703888 |
Synonyms: |
CCKGAD1TG, Tg(Cck-eGFP/miRNA:GAD1)2KM |
Transgene: |
Tg(Cck-EGFP/RNAi:Gad1)2Mirn Location: unknown
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Alliance: |
Tg(Cck-EGFP/RNAi:Gad1)2Mirn page
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Transgene Type: |
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Transgenic (Conditional ready, Knockdown, Reporter) |
Mutation: |
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Insertion
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Tg(Cck-EGFP/RNAi:Gad1)2Mirn involves 1 genes/genome features (Gad1)
View all
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Mutation details: The transgenic construct uses 116 kbp from the bacterial artificial chromosome (BAC) RP23-60I1 containing the cholecystokinin locus (Cck) as well as ~55 kbp of centromeric sequences (including a portion of the Trak1 locus) and ~56 kbp of telomeric sequences; no other confirmed complete genes are present in the flanking sequences. Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Cck: eGFP, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. No other loci on the BAC were altered.
(J:227900)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:227900 Schmidt MJ, et al., Modulation of behavioral networks by selective interneuronal inactivation. Mol Psychiatry. 2014 May;19(5):580-7 |
All: |
1 reference(s) |
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