Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn
Transgene Detail

Summary

• Symbol: Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn
• Name: transgene insertion 3, Karoly Mirnics
• MGI ID: MGI:5703933
• Synonyms: Pvalb/Gad1
• Transgene: Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn Location: unknown
• Alliance: Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Conditional ready, Knockdown, Reporter)
• Mutation: Insertion
• Tg(Pvalb-tdTomato/RNAi:Gad1)3Mirn involves 1 genes/genome features (Gad1) View all
• Mutation details: The transgenic construct uses 129.7 kbp from the bacterial artificial chromosome (BAC) RP24-306A6 possessing the parvalbumin locus (Pvalb) as well as ~65 kbp of centromeric sequences (including the complete Ift27 locus) and ~50 kbp of telomeric sequences (including the complete Ncf4 locus). Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Pvalb: the red fluorescent protein tdTomato coding sequence, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. Also in vitro, the sequences encompassing exons 2-4 of the Ift27 locus were deleted. No other loci on the BAC were altered. (J:227902)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:227902 Brown JA, et al., Inhibition of parvalbumin-expressing interneurons results in complex behavioral changes. Mol Psychiatry. 2015 Dec;20(12):1499-507
• All: 2 reference(s)