Ap1m2^tm1.1Ohno
Targeted Allele Detail

Summary

• Symbol: Ap1m2^tm1.1Ohno
• Name: adaptor protein complex AP-1, mu 2 subunit; targeted mutation 1.1, Hiroshi Ohno
• MGI ID: MGI:5704433
• Synonyms: Ap1m2^-, deltaEX4,5
• Gene: Ap1m2 Location: Chr9:21206753-21223617 bp, - strand Genetic Position: Chr9, 7.76 cM
• Alliance: Ap1m2^tm1.1Ohno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226582
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was inserted upstream of exon 4 and a loxP-flanked neomycin selection cassette was inserted downstream of exon 5. Cre-mediated recombination resulted in the deletion of the loxP-flanked exons 4 and 5 as well as the neomycin selection cassette. RT-PCR analysis demonstrated that no detectable transcript was present in the small intestinal epithelium of homozygous mutant mice. (J:226582)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ap1m2 Mutation: 23 strains or lines available

References

• Original: J:226582 Hase K, et al., AP-1B-mediated protein sorting regulates polarity and proliferation of intestinal epithelial cells in mice. Gastroenterology. 2013 Sep;145(3):625-35
• All: 3 reference(s)